Substance present in ayahuasca brew stimulates generation of human neural cells


Main article text

Introduction

Material and Methods

Chemicals

Human pluripotent stem cells

Human neural progenitor cells

High content screening

High content analysis

Immunocytochemistry

Statistical analysis

Results

Harmine increases proliferation of human neural progenitors: a possible role of DYRK1A

Discussion

Supplemental Information

Supplementary Figures 1–3

DOI: 10.7717/peerj.2727/supp-1

Raw data of all figures

DOI: 10.7717/peerj.2727/supp-2

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Vanja Dakic conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Renata de Moraes Maciel conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, reviewed drafts of the paper.

Hannah Drummond performed the experiments, reviewed drafts of the paper.

Juliana M. Nascimento prepared figures and/or tables, reviewed drafts of the paper.

Pablo Trindade wrote the paper, reviewed drafts of the paper.

Stevens K. Rehen conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Data Availability

The following information was supplied regarding data availability:

The raw data has been supplied as Data S1.

Funding

This study was funded by the following Brazilian funding agencies: National Council for Scientific and Technological Development (CNPq), Foundation for Research Support in the State of Rio de Janeiro (FAPERJ), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Funding Authority for Studies and Projects (FINEP), Brazilian Development Bank (BNDES) and São Paulo Research Foundation (grant 2014/21035-0). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 

Source: https://peerj.com

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